Speaker
Description
Transporter-mediated drug delivery is a crucial factor in pharmacokinetic and pharmacodynamic (PK/PD) studies. Transporter kinetics are typically evaluated using the Michaelis–Menten (MM) model, which estimates the maximum reaction rate($V_{\max}$) and the Michaelis constant($K_M$). However, the MM model assumes a well-mixed environment, whereas transporters operate in localized spatial settings, which can lead to estimation inaccuracies. While previous studies addressed this spatial discrepancy, they introduced an additional spatial parameter that is difficult to determine experimentally. To overcome this practical limitation, we reformulated the reaction–diffusion equations in terms of more accessible parameters. Furthermore, we rigorously evaluated the accuracy and practical identifiability of these transformed parameters with real experimental data. By transitioning to practically measurable variables, this study bridges the gap between complex spatial models and empirical laboratory data, offering a robust and experimentally interpretable framework for analyzing transporter kinetics.
